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KMID : 0861020140290040083
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Korea Journal of Herbology
2014 Volume.29 No. 4 p.83 ~ p.89
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Anti-inflammatory Effect of Houttuyniae Herba Water Extract on LPS-induced RAW 264.7 Mouse Macrophages
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Hwang In-Seung
Kim Young-Jin
Park Yun-Soo
Kim Hyung-Joo
Kim Do-Hoon
Park Wan-Su
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Abstract
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Objectives : The purpose of this study was to investigate effects of Houttuyniae Herba water extract (HC) on calcium release and production of various inflammatory mediators such as nitric oxide (NO), interferon-inducible protein (IP)-10, platelet derived growth factor (PDGF)-BB, keratinocyte-derived chemokine (KC), vascular endothelial growth factor (VEGF), interleukin (IL)-4, and IL-5 in lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages.
Methods : NO production was measured by Griess reagent assay. Intracellular calcium level was measured with Fluo-4 assay. Levels of cytokines were measured by High-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology.
Results : HC significantly decreased NO production for 24 hrs incubation at the concentrations of 10, 25, 50, 100, and 200 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). HC significantly decreased production of IP-10, KC, VEGF, and PDGF-BB for 24 hrs incubation at the concentrations of 50, 100, and 200 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). HC also significantly decreased intracellular calcium release for 24 hrs incubation at the concentrations of 25, 50, 100, and 200 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). But HC did not show any significant effect on production of IL-4 and IL-5 in LPS-induced RAW 264.7.
Conclusions : The results suggested that HC has anti-inflammatory property related with its inhibition on the production of NO, IP-10, KC, VEGF, and PDGF-BB in LPS-induced macrophages via calcium pathway
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KEYWORD
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Houttuyniae Herba, Macrophage, Inflammation, Cytokine, Nitric oxide, Calcium
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